Fine Structure Immunocytochemistry

Immunocytochemistry is one of the most powerful techniques employed in defining cellular location and analyzing molecular and cellular organizations. It includes the preparation and fixation of cells without adversely affecting the visualization of subcellular structures by usage of highly specific reagents - predominantly labelled antibodies - and electron microscopy analysis. The book describes all practical steps involved in the transition of living cells to a labelled thin section in the electron microscope. It includes theoretical background to allow lab workers to modify and apply the procedures to their particular nature of work and to interpret microscopical results. Stereological methods for quantitative analysis are also covered. The work helps to obtain a realistic picture of the organization of a living cell.