Flow Cytometry and Cell Sorting

Flow cytometry.Operation of a flow cytometer. Immunofluorescence. Conjugation of fluorochomes, haptens, and phycobiliproteins to antibodies. Immunofluorescence: basic considerations. Multicolor immunofluorescence analysis. Combined intracellular and surface staining. Scatchard analysis bu flow cytometry. DNA and proliferation. Preparation and staining of cells for high-resolution DNA analysis. Simultaneous flow cytometric detection of bromodexyuridine incorporation and cell surface marker expression. High-resolution cell cycle analysis. The flow cytometric bromedeoxyuridine-hoechst quenching technique. Cellular activation and biochemistry. Cell activation. Indo-1 ratiometric analysis of intracellular lonized calcium. Biochemical parameters of cell function. Molecular analysis of transcriptional control. The FACS-galactosidase assay. Ligand acidification by nodadherent cells. Cel sorting. Powerful preselection. High of a flow sorter. Sorting of rare cells. Fluorescence-activated chromosome sorting. Flow cytometry and sorting of plant chromosomes. large particle sorting. Safety. biological and laser safety.