Specificity and Function of Clonally Developing T Cells (Current Topics in Microbiology and Immunology)

The international workshop on "Specificity and Function of Clonally Developing T Cells" was held at SchloG Rei sensburg (near UIm, West Germany) on March 17-20, 1985. The meeting brought together immunologists study ing clonal T-cell development in man and mouse in various in vitro systems at the cellular as well as molecular level. It was an attempt to provide an overview of the current research interests of groups working on (a) the developmen tal potential of in vitro expanding primary T-cell clones (investigated using limiting dilution analysis) and cloned T -cell lines established in long-term culture ; (b) the signals required for the expression of particular patterns of (func tional and antigen receptor) phenotypes by T cells which are either freshly explanted in vitro, or maintained in vitro as cloned long-term lines; and (c) the generation of an MHC-restricted T-cell repertoire. In the study of thymocytes emphasis has shifted towards the presumably immature adult/embryonic subset(s) which is (are) devoid of subset-specific differentiation markers (L3T4, Lyt-2). Neither the signal requirement(s) for clonal expansion in vitro of these cells, nor their precursor role for any functional T -cell lineage are as yet unambiguously established. The multiple modes of human T-cell activation (e.g., via Tp44, T11, T3/Ti molecular complexes) were em phasized by a number of presentations and raised the ques tion of whether these different modes of activation induce different functional activities in individual T-cell clones.