Nonradioactive Labeling and Detection of Biomolecules

All the bioanalytical labeling and detection techniques inone source! This book gathers together all the importantnonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids likeDigoxigenin: Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, SilverEnhancement, and Synthetic Nucleic Acid Probe (SNAP) as wellas the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescentdetection.Additionally, applications for the use of non-isotopicallylabeled biomolecules are described. Specific protocols aregiven for hybridization analysis such as blot, colony/plaqueand in-situ hybridization formats, quantitative formats, andalso nonradioactivetechniques for nucleic acid sequencingand amplification.Each chapter contains a short introduction, a detaileddescription of the method with labprotocols, troubleshooting tips and references.