Cryotechniques in Biological Electron Microscopy

This book is intended as a versatile handbook with comprehensive articles on cryotechniques in biological electron microscopy. Each chapter provides a selective and critical review on a topic of major interest, written by a well known expert. Three fundamental articles treat the physics of water and ice, the physics of specimen preparation, and the effects of radiation damage. Aspects of the cryofixation of dynamic events and of defined physiological states are also considered. A recapitulation of safety rules completes the volume. This handbook provides a good overview of the state-of-the-art for the newcomer as well as dor more established workers in the field.