Enzymology and Molecular Biology of Carbonyl Metabolism 5

1. Site Directed Mutagenesis to Probe for Active Site Components of Liver Mitochondrial Aldehyde Dehydrogenase.- 2. Substrate Binding Pocket Structure of Human Aldehyde Dehydrogenases: A Substrate Specificity Approach.- 3. Human Class 1 Aldehyde Dehydrogenase: Expression and Site-Directed Mutagenesis.- 4. Nitrate Esters as Inhibitors and Substrates of Aldehyde Dehydrogenase.- 5. Use of a Chromophoric Reporter Group to Probe the Active Site of Cytosolic Aldehyde Dehydrogenase.- 6. Studies of the Esterase Activity of Cytosolic Aldehyde Dehydrogenase Using Sterically Hindered and Cyclic Substrates.- 7. The Reduction of Propionic Anhydride by Aldehyde Dehydrogenase-Nadh Mixtures at pH 7.- 8. Cloning and Characterisation of the cDNA for Sheep Liver Cytosolic Aldehyde Dehydrogenase.- 9. Crystallization of Sheep Liver Cytosolic Aldehyde Dehydrogenase in a Form Suitable for High Resolution X-Ray Structural Analysis.- 10. Progress toward the Tertiary Structure of (Class 3) Aldehyde Dehydrogenase.- 11. UDP-Glucose Dehydrogenase: Structural Characteristics.- 12. Kinetic Studies on Class 3 Aldehyde Dehydrogenase from Bovine Cornea.- 13. Covalent Modification of Class 2 and Class 3 Aldehyde Dehydrogenase by 4-Hydroxynonenal.- 14. Constitutive and Overexpressed Human Cytosolic Class-3 Aldehyde Dehydrogenases in Normal and Neoplastic Cells/Secretions.- 15. Metabolism of Cyclophosphamide by Aldehyde Dehydrogenases.- 16. Tissue-Specific Expression and Preliminary Functional Analysis of the 5? Flanking Regions of the Human Mitochondrial Aldehyde Dehydrogenase (ALDH2) Gene.- 17. Transgenesis of the Aldehyde Dehydrogenase-2 (ALDH2) Locus in a Mouse Model and in Cultured Human Cells.- 18. Class 3 Aldehyde Dehydrogenase: A Northern Perspective in the Land Down Under.- 19. Studies on the Induction of Rat Class 3 Aldehyde Dehydrogenase.- 20. Mouse Class 3 Aldehyde Dehydrogenases.- 21. Cloning and Characterization of Genes Encoding Four Additional Human Aldehyde Dehydrogenase Isozymes.- 22. New Human Aldehyde Dehydrogenases.- 23. Retinoic Acid Synthesizing Enzymes in the Embryonic and Adult Vertebrate.- 24. Retinoic Acid Synthesis in the Developing Spinal Cord.- 25. Structure and Mechanism of Aldehyde Reductase.- 26. Expression of Human and Rat Carbonyl Reductase in E. coli: Comparison of the Recombinant Enzymes.- 27. Molecular Cloning and Sequencing of Mouse Hepatic 11ß-Hydroxysteroid Dehydrogenase/Carbonyl Reductase: A Member of the Short Chain Dehydrogenase Superfamily.- 28. Molecular Modelling Calculations on the Binding of D- and L-Xylose to Wild-Type Aldose Reductase and Its H11OQ and H11OA Mutants.- 29. Stopped-Flow Studies of Human Aldose Reductase Reveal which Enzyme Form Predominates during Steady-State Turnover in Either Reaction direction.- 30. Lysine Residues in the Coenzyme-Binding Region of Mouse Lung Carbonyl Reductase.- 31. Substrate Specificity and Kinetic Mechanism of Tetrahymena 20?-Hydroxysteroid Dehydrogenase.- 32. Purification and Characterization of Recombinant Human Placental and Rat Lens Aldose Reductases Expressed in Escherichia coli.- 33. Rat and Human Bile Acid Binders Are Members of the Monomeric Reductase Gene Family.- 34. The Alcohol Dehydrogenase System.- 35. Promoters of the Mammalian Class III Alcohol Dehydrogenase Genes.- 36. Class I and Class Iv Alcohol Dehydrogenase (Retinol Dehydrogenase) Gene Expression in Mouse Embryos.- 37. Molecular Evolution of Class I Alcohol Dehydrogenases in Primates: Models for Gene Evolution and Comparison of 3? Untranslated Regions of cDNAS.- 38. the Role of Leucine 116 in Determining Substrate Specificity in Human B1 Alcohol Dehydrogenase.- 39. Mutations of Human Class III Alcohol Dehydrogenase.- 40. Human and Rat Class IV Alcohol Dehydrogenases: Correlations of Primary Structures with Enzymatic Properties.- 41. Cloning and Expression of a Human Stomach Alcohol Dehydrogenase Isozyme.- 42. Purification and Properties of Murine Corneal Alcohol Dehydrogenase: Evidence for Class IV ADH P